1 resultado para CLOT LYSIS
em Greenwich Academic Literature Archive - UK
Filtro por publicador
- ABACUS. Repositorio de Producción Científica - Universidad Europea (1)
- Aberystwyth University Repository - Reino Unido (1)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (1)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (1)
- Aquatic Commons (7)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (13)
- Aston University Research Archive (6)
- B-Digital - Universidade Fernando Pessoa - Portugal (3)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (10)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (4)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (5)
- Bioline International (2)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (74)
- Brock University, Canada (4)
- Bucknell University Digital Commons - Pensilvania - USA (1)
- CaltechTHESIS (3)
- Cambridge University Engineering Department Publications Database (2)
- CentAUR: Central Archive University of Reading - UK (33)
- Centro Hospitalar do Porto (1)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (18)
- Cochin University of Science & Technology (CUSAT), India (1)
- CORA - Cork Open Research Archive - University College Cork - Ireland (1)
- Deakin Research Online - Australia (10)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (1)
- Digital Commons at Florida International University (6)
- Digital Knowledge Repository of Central Drug Research Institute (1)
- DigitalCommons - The University of Maine Research (1)
- DigitalCommons@The Texas Medical Center (13)
- DigitalCommons@University of Nebraska - Lincoln (2)
- DRUM (Digital Repository at the University of Maryland) (2)
- Duke University (2)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (2)
- eScholarship Repository - University of California (1)
- Gallica, Bibliotheque Numerique - Bibliothèque nationale de France (French National Library) (BnF), France (2)
- Glasgow Theses Service (2)
- Greenwich Academic Literature Archive - UK (1)
- Helda - Digital Repository of University of Helsinki (11)
- Hospitais da Universidade de Coimbra (1)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (1)
- Indian Institute of Science - Bangalore - Índia (20)
- Institutional Repository of Leibniz University Hannover (1)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (1)
- Memoria Académica - FaHCE, UNLP - Argentina (2)
- Ministerio de Cultura, Spain (11)
- National Center for Biotechnology Information - NCBI (45)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (7)
- Portal de Revistas Científicas Complutenses - Espanha (1)
- Publishing Network for Geoscientific & Environmental Data (4)
- QSpace: Queen's University - Canada (7)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (22)
- Queensland University of Technology - ePrints Archive (17)
- Repositório Científico da Universidade de Évora - Portugal (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (1)
- Repositório Institucional da Universidade Federal do Rio Grande - FURG (2)
- Repositório Institucional da Universidade Tecnológica Federal do Paraná (RIUT) (3)
- Repositorio Institucional de la Universidad Pública de Navarra - Espanha (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (169)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (4)
- SAPIENTIA - Universidade do Algarve - Portugal (3)
- Universidad Autónoma de Nuevo León, Mexico (1)
- Universidad de Alicante (3)
- Universidad del Rosario, Colombia (7)
- Universidad Politécnica de Madrid (4)
- Universidade dos Açores - Portugal (2)
- Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP) (1)
- Universidade Federal de Uberlândia (2)
- Universidade Federal do Pará (3)
- Universidade Federal do Rio Grande do Norte (UFRN) (4)
- Universita di Parma (2)
- Universitat de Girona, Spain (2)
- Université de Lausanne, Switzerland (2)
- Université de Montréal, Canada (11)
- University of Michigan (19)
- University of Queensland eSpace - Australia (18)
- University of Washington (4)
- WestminsterResearch - UK (3)
- Worcester Research and Publications - Worcester Research and Publications - UK (1)
Resumo:
Linear poly(amidoamine)s (PAAs) have been designed to exhibit minimal non-specific toxicity, display pH-dependent membrane lysis and deliver genes and toxins in vitro. The aim of this study was to measure PAA cellular uptake using ISA1-OG (and as a reference ISA23-OG) in B16F10 cells in vitro and, by subcellular fractionation, quantitate intracellular trafficking of (125)I-labelled ISA1-tyr in liver cells after intravenous (i.v.) administration to rats. The effect of time after administration (0.5-3h) and ISA1 dose (0.04-100mg/kg) on trafficking, and vesicle permeabilisation (N-acetyl-b-D-glucosaminidase (NAG) release from an isolated vesicular fraction) were also studied. ISA1-OG displayed approximately 60-fold greater B16F10 cell uptake than ISA23-OG. Passage of ISA1 along the liver cell endocytic pathway caused a transient decrease in vesicle buoyant density (also visible by TEM). Increasing ISA1 dose from 10mg/kg to 100mg/kg increased both radioactivity and NAG levels in the cytosolic fraction (5-10 fold) at 1h. Moreover, internalised ISA1 provoked NAG release from an isolated vesicular fraction in a dose-dependent manner. These results provide direct evidence, for the first time, of PAA permeabilisation of endocytic vesicular membranes in vivo, and they have important implications for potential efficacy/toxicity of such polymeric vectors.